Fig. 4: Cancer cells and T-cell engagement via bispecific antiCAFs-DMS-AptT in 3D and 2D models. | Nature Communications

Fig. 4: Cancer cells and T-cell engagement via bispecific antiCAFs-DMS-AptT in 3D and 2D models.

From: Pancreatic tumor eradication via selective Pin1 inhibition in cancer-associated fibroblasts and T lymphocytes engagement

Fig. 4

a Schematic workflow of Pan02 spheroids and CAFs indirect co-culture. b Representative morphologic images of Pan02 spheroids at the end of the indirect co-culture. Scale bars are 100 μm. c Dynamic growth curves of Pan02 spheroids during the indirect co-culture (n = 5 biologically independent spheroids; Error bars, mean ± SD). Two-tailed Student’s t tests. d Schematic workflow of the incubation of MOHITO cells with Cy5-labeled antiCAFs-DMS-AptT. e Quantitative analysis of the binding of Cy5-labeled antiCAFs-DMS or antiCAFs-DMS-AptT to MOHITO cells via flow cytometry (Three independent repetitions got similar results.). f Schematic illustration of the direct co-culture of CAFs and MOHITO cells. g Representative microscopic images of the co-culture experiment. Three independent repetitions got similar results. Scale bars are 10 μm. Source data are provided as a Source Data file.

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