Fig. 2: Construction of engineered phage-resistant (EPR) E. coli strains.
From: Systematic strategies for developing phage resistant Escherichia coli strains
a–c Phage plaque assays were used to compare the phage infection efficiencies in WT-MG1655, MG1655 carrying pWHU3640, MG1655-PT and MG1655-EPR strains (a); in WT-W3110, W3110 carrying pWHU3640, W3110-PT and W3110-EPR strains (b); and in WT-MG1655 (DE3), MG1655 (DE3) carrying pWHU3640, MG1655 (DE3)-PT and MG1655 (DE3)-EPR strains (c). The results are representative of three independent experiments. d The systematic genome engineering strategy confers strong phage resistance on different E. coli hosts. EOPs were determined to assess the protection levels of E. coli-PT and E. coli-EPR strains against coliphages. All the data were obtained at least three times. Source data are provided as a Source Data file.
