Fig. 1: CaMK4 expression is increased in psoriatic lesional skin from patients with psoriasis and mice with IMQ-induced psoriasis.

a Representative images of immunohistochemical staining for CaMK4 in human paired psoriatic lesional skin and non-lesional skin (n = 4) and healthy skin (n = 7). Scale bar = 100 μm. b The mRNA level of CAMK4 in the cells of peripheral blood from healthy controls (HCs, n = 24) and patients with psoriasis (Ps, n = 24). c The proportion of CaMK4⁺ cells and the MFI of CaMK4 in peripheral T cell subsets and CD14⁺ monocytes from healthy controls (n = 15) and patients with psoriasis (n = 12). d The relationships between the expression of pro-inflammatory cytokines and CAMK4 in the cells of peripheral blood from patients with psoriasis (n = 24). e Representative images of CaMK4-stained mouse skin sections (n = 3 biologically independent samples). Scale bar = 50 μm. Vas, vaseline. f Western blot analysis of CaMK4 expression in primary KCs and MACS-sorted F4/80⁺ cells and CD11c⁺ cells from mouse skin (n = 3 biologically independent samples). g Quantitative PCR analysis of Camk4 expression as indicated (n = 3 biologically independent samples). Five to seven mouse skin tissues were pooled as one sample for MACS sorting, and the purity of sorted cells was >90% (f, g). Data are shown as mean ± SD. For (b, c), two-sided Mann–Whitney test; for (d), linear regression analysis; for (g), two-sided unpaired Student’s t test. Source data are provided as a Source Data file.