Fig. 4: Dermal MHC II+ macrophages are the main source of IL-10 in IMQ-treated Camk4−/− mice. | Nature Communications

Fig. 4: Dermal MHC II+ macrophages are the main source of IL-10 in IMQ-treated Camk4−/− mice.

From: Calcium/calmodulin-dependent protein kinase IV promotes imiquimod-induced psoriatic inflammation via macrophages and keratinocytes in mice

Fig. 4

a Quantitative PCR analysis of Il10 expression in mouse skin. b Mouse skin sections were stained for IL-10. Scale bar = 200 μm. c Immunofluorescent microscopy images of mouse skin sections stained with anti-F4/80 (green), anti-IL-10 (red), and DAPI (blue). The solid arrows show F4/80+IL-10+ cells, and the dashed arrows show F4/80+IL-10− cells in the dermis. Scale bar = 50 μm. d The expression of IL-10 in F4/80+CD11c+ cells, CD11b+ DCs, and MHC II+ macrophages as determined by flow cytometry. e Statistical analysis of the MFI of IL-10. a–e N = 8 per group from two independent experiments. Data are shown as mean ± SD. For (a), two-sided Mann–Whitney test; for (e), two-sided unpaired Student’s t test. Source data are provided as a Source Data file.

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