Fig. 5: CaMK4 inhibits IL-10 production by macrophages through the ADCY1-cAMP-Erk1/2 and p38 pathways. | Nature Communications

Fig. 5: CaMK4 inhibits IL-10 production by macrophages through the ADCY1-cAMP-Erk1/2 and p38 pathways.

From: Calcium/calmodulin-dependent protein kinase IV promotes imiquimod-induced psoriatic inflammation via macrophages and keratinocytes in mice

Fig. 5

a Co-IP assay of CaMK4 and ADCY1 in BMDMs. b Western blot analysis of CaMK4, ADCY1, p-Erk1/2, Erk1/2, p-p38, and p38 in BMDMs treated with IMQ, KN-93, ST034307, or cAMP. c Quantitative PCR analysis of Il10 expression as indicated (n = 3 biologically independent samples). d ELISA analysis of IL-10 in the supernatants of BMDMs as indicated (n = 3 biologically independent samples). e Quantitative PCR analysis of macrophage phenotype markers (M1 and M2) in Camk4+/+ and Camk4−/− BMDMs stimulated with IMQ (n = 4 biologically independent samples). f Quantitative PCR analysis of phagocytosis-related genes (n = 4 biologically independent samples). g Western blot analysis of CaMK4, p-Erk1/2, Erk1/2, p-p38, and p38 in MACS-sorted peripheral CD14+ monocytes from patients with psoriasis. h Quantitative PCR analysis of IL10 in monocytes (n = 3 biologically independent samples). i ELISA analysis of IL-10 in monocyte supernatants (n = 3 biologically independent samples). j Quantitative PCR analysis of pro-inflammatory genes in monocytes (n = 3 biologically independent samples). The experiments in a–f were repeated three times with similar results. Data are shown as mean ± SD. For (e, f, j), two-sided unpaired Student’s t test; for (h, i), one-way ANOVA with Bonferroni’s post-test. Source data are provided as a Source Data file.

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