Fig. 2: Transcription characteristics and heterogeneity among the six FB subclusters.
From: Single-cell transcriptome atlas of the human corpus cavernosum
a tSNE plots of the six fibroblast (FB) subclusters. Cells are coloured according to their types (left panel) or expression level of the marker gene (right panel). b Bubble diagram of the top five DEGs in each FB subcluster. A gradient of grey to red indicates low to high expression levels. The size of bubbles indicates the percentage of cells positive for DEGs in each subcluster. c Heatmap showing the DEGs in each FB subcluster (left panel) based on normal samples. The GO terms (biological process) associated with their DEGs are listed in the right panel. The count of DEGs for each FB subcluster are listed in the middle panel. d Bar plot showing the cell count proportion of each FB subcluster in normal and ED CC. e Cell function (proliferation and death) predicted by IPA analysis according to the DEGs of each FB subcluster shown as a bubble diagram. A gradient of light blue to red indicates inhibition to activation of the term. The size of the bubble indicates the P-value. f Violin plot combined with box plot showing the expression score of the three major energy metabolism pathway genes in each FB subcluster. Box plots indicate median (middle line), 25th, 75th percentile (box) and 5th and 95th percentile (whiskers) as well as outliers (single points).
