Fig. 6: FASN and the Lands cycle are required to deflect ferroptosis in KMLC.
From: Targeting de novo lipogenesis and the Lands cycle induces ferroptosis in KRAS-mutant lung cancer
a–d C11-BODIPY staining in KM, KRAS-WT, and H522-KM LC cells. Oxidized and reduced C11-BODIPY are indicated in red and green, respectively. Bars indicate the relative C11-BODIPY oxidation (n, number of cells). e Schematic of the GPX4 axis of ferroptosis and some of its regulators. Red = pro-ferroptosis; Green = anti-ferroptosis. NAC, N-acetyl cysteine; GSH, glutathione; GSSG, glutathione disulfide; ML162, GPX4 inhibitor; Fer-1, ferrostatin-1; PUFA, Polyunsaturated fatty acids; OxPUFA, oxidized PUFA; PL, phospholipids; MUFA, monounsaturated fatty acids; SFA, saturated fatty acids; LA, linoleic acid; PC, phosphatidylcholine. f, g C11-BODIPY rescue experiments in the indicated cell lines and their quantification. h, i C11-BODIPY stain on A549 cell-line transfected with the indicated siRNAs (48 h post transfection) and their quantification. In g and i n of cells analyzed over two independent experiments are indicated in the graphs. Bars represent mean ± SD. In b and d two-tailed unpaired student t-test, in g multiple t-tests.
