Fig. 1: Elevated numbers of tubes and sprouts formed by TECs as compared to NECs and more rapid tube formation in TECs. | Nature Communications

Fig. 1: Elevated numbers of tubes and sprouts formed by TECs as compared to NECs and more rapid tube formation in TECs.

From: Integrin α3β1 promotes vessel formation of glioblastoma-associated endothelial cells through calcium-mediated macropinocytosis and lysosomal exocytosis

Fig. 1

a, b NECs (422) and TECs (isolate ccf2687) were cultured for 48 h in collagen gels as described in Methods. Representative images are shown in a, b is the corresponding statistical analysis by two-sided Wilcoxon rank-sum tests. Yellow arrows denote tubes. n = 6 different fields in each group. c, d NECs (623) and TECs (isolate ccf2445) were cultured on Matrigel for 24 h or 48 h. Representative images are shown in c, d is the corresponding statistical analysis by two-sided Wilcoxon rank-sum tests. Yellow arrows denote tubes and red arrows denote sprouts. For tube numbers, n = 12 different fields in each group; and for sprout numbers, n = 6 different fields in each group. Red and black bars in b, d indicate means with 95% confidence intervals. e Live video microscopy of NECs (376) and TECs (isolate ccf2515) over the first 9 h of culture on Matrigel. Representative images are shown. f Immunofluorescent staining of laminin α5 chain (sc-16592; Alexa-Fluor-594; red) in the basement membrane of tubes formed by NECs (376 and 422) and TECs (isolates ccf2445 and ccf2566) on Matrigel for 12 and 24 h. Cell nuclei stained with DAPI (blue). Independent experiments were performed at least two times with similar results. Source data are provided as a source data file.

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