Fig. 1: Expression of RalGAP−Ral signaling components in the heart and cardiomyocytes.
From: The RalGAPα1–RalA signal module protects cardiac function through regulating calcium homeostasis
A, B Protein expression of RalGAP complexes and their downstream Ral small G proteins in the hearts of 2-month-old WT male mice that were subjected to TAC or sham operation for 12 days. GAPDH was used as a loading control. B quantitation of blots shown in A. n = 4 (Sham) and 5 (TAC). p = 0.0011 (RalGAPα1), 0.843 (RalGAPα2), 0.0055 (RalGAPβ), 0.113 (RalA) and 0.386 (RalB). C mRNA expression of RalGAP complexes and their downstream Ral small G proteins in the heart of 2-month-old WT male mice that were subjected to TAC or sham operation for 12 days. n = 4 (Sham) and 5 (TAC). p = 0.578 (Ralgapα1), 0.138 (Ralgapα2), 0.792 (Ralgapβ), 0.112 (RalA) and 0.457 (RalB). D, E Protein expression of RalGAPα1/β complexes and β-MHC in NRVCs stimulated with or without norepinephrine (NE). E quantitation of blots shown in D. n = 4. p = 0.035 (RalGAPα1) and 0.0078 (RalGAPβ). F mRNA expression of RalGAP complexes in NRVCs stimulated with or without NE. n = 4 (Control) and 5 (NE). p = 0.262 (Ralgapα1) and 0.877 (Ralgapβ). G, H Protein expression of RalGAPα1/β complexes and β-MHC in NRVCs stimulated with or without Angiotensin II (Ang II). H, quantitation of blots shown in G. n = 4. p =0.0059 (RalGAPα1) and 0.039 (RalGAPβ). I mRNA expression of RalGAP complexes in NRVCs stimulated with or without Ang II. n = 6. p = 0.085 (Ralgapα1) and 0.290 (Ralgapβ). The data are given as the mean ± SEM. Statistical analyses were carried out using two-sided t-test. One-asterisk indicates p < 0.05, and two-asterisk indicates p < 0.01. Source data are provided as a Source Data file.
