Fig. 3: LNP-mediated local intrahepatic pDNA delivery in cluster-mode screening. | Nature Communications

Fig. 3: LNP-mediated local intrahepatic pDNA delivery in cluster-mode screening.

From: Multi-step screening of DNA/lipid nanoparticles and co-delivery with siRNA to enhance and prolong gene expression

Fig. 3

a Scheme for intrahepatic delivery. b Whole-body bioluminescence flux of female BALB/c mice (6–8 weeks) at 12 h following a single intrahepatic injection of different LNP formulations containing 3 μg of pDNA (50% Luc+ 50% mCherry) per mouse (n = 2, 48 for the whole experiment). Ex vivo c imaging and d quantitative flux of luminescence in the liver at 12 h post-administration. e FACS was used to quantify the percentage of specific cell types in the liver expressing mCherry for the top 12 clusters. Data are presented as mean±S.D. (n = 2 mice per group). Source data are provided as a Source Data file.

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