Fig. 1: CLK4 regulates cardiomyocyte hypertrophy, and its expression decreases with disease. | Nature Communications

Fig. 1: CLK4 regulates cardiomyocyte hypertrophy, and its expression decreases with disease.

From: CDC-like kinase 4 deficiency contributes to pathological cardiac hypertrophy by modulating NEXN phosphorylation

Fig. 1

a qPCR detection of the expression of Nppa and Nppb in NRVMs transfected with control or Clk1-4 siRNAs. b, c Representative image of NRVMs treated with control or Clk4 siRNA for 48 h with cell area quantification. Scale bar: 50 μm. d Western blot analysis of CLK4 protein expression in failed mouse hearts subjected to either transverse aortic constriction (upper) or ISO infusion (lower) and in corresponding control mouse hearts. GAPDH served as loading control. e Quantification of the western blots in d. Ctrl control, NRVMs neonatal rat ventricular myocytes, HF heart failure, TAC transverse aortic constriction, ISO isoproterenol. a n = 3 biologically independent samples per group; c, n = 4 biologically independent samples per group; e, n = 3 animals per group. a statistical analysis was performed using one-way ANOVA and Dunnett multiple comparisons test; for c and e, statistical analyses were performed using upaired, two-tailed Student’s t test. Data are presented as the means ± S.E.M.; P values are shown in each graph. Source data are provided as a Source Data file.

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