Fig. 2: ZFP36L1, REST, YAP1, and WWTR1 are bound by LSD1 and de-repressed by LSD1 inhibition.
From: Regulation of neuroendocrine plasticity by the RNA-binding protein ZFP36L1
a RT-qPCR of NCI-H1876 cells treated with ORY-1001 (1 nM) or ORY-1001 (1 nM) + KDM5-C70 (1 μM) for 6 days. n = 4 biological independent experiments. b, d–g RT-qPCR for ASCL1 (b), ZFP36L1 (d), REST (e), YAP1 (f), and WWTR1 (g) in different human SCLC cell lines treated with ORY-1001 (100 nM) or DMSO for 6 days. For b, n = 3 biological independent experiments. For d–g, n = 3 biological independent experiments for all cell lines except CORL47 and NCI-H1876 where n = 4 biological independent experiments. c Cellular proliferation of human SCLC cell lines treated with ORY-1001 (100 nM) for 6 days relative to the cells treated with DMSO. n = 3 biological independent experiments. h, i ChIP-qPCR of NCI-H1876 (h) and CORL47 (i) cells treated with ORY-1001 (100 nM) or DMSO for 6 days followed by immunoprecipitation (IP) for LSD1 and qPCR for the genes indicated. See Supplementary Fig. 3a–d for the identification of enrichment peaks in the genes where LSD1 is bound. For h, i, n = 4 biological independent experiments for all LSD1 ChIP experiments and n = 2 biological independent experiments for all control IgG ChIP experiments. For all panels, data are presented as mean values +/– SEM. Statistical significance was calculated using unpaired, two-tailed students t-test and p-values are indicated on each figure panel.
