Fig. 7: Analysis of AM0627 and BT4244 activity with isolated secreted MUC1-6.5xTRs reporters. | Nature Communications

Fig. 7: Analysis of AM0627 and BT4244 activity with isolated secreted MUC1-6.5xTRs reporters.

From: Structural and mechanistic insights into the cleavage of clustered O-glycan patches-containing glycoproteins by mucinases of the human gut

Fig. 7

SDS–PAGE analysis of wt AM0627A21-E506 and BT4244 (dose titration) digestion of secreted purified MUC1-6.5xTRs reporters produced in glycoengineered HEK293 cells (WT, mSTa, T, and Tn O-glycoforms). Purified reporters (0.5 µg) were incubated for 3 h at 37 °C with indicated enzyme and gels visualized with Krypton fluorescent protein stain. * indicates the bands corresponding to wt AM0627A21-E506 and BT4244. The experiments were performed in duplicate.

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