Fig. 1: Efficacy of NHC against VOC in cultured cells and primary HAEs. | Nature Communications

Fig. 1: Efficacy of NHC against VOC in cultured cells and primary HAEs.

From: SARS-CoV-2 VOC type and biological sex affect molnupiravir efficacy in severe COVID-19 dwarf hamster model

Fig. 1

a NHC dose-response assays against SARS-CoV-2 WA1 and VOC alpha, beta, gamma, delta, and omicron on VeroE6-TMPRSS2 cells. EC50 values from non-linear regression modeling are shown with 95% confidence intervals in parenthesis. b Activity of 10 µM basolateral NHC administered against VOC as in a on well-differentiated primary HAE organoids. Apically shed virus was harvested every 24 hours during a 3-day period after infection. a, b represent independent biological repeats, lines intersect group means. LoD limit of detection. c Confocal microscopy of primary HAE organoids infected with SARS-CoV-2 VOC gamma. Basolateral NHC suppresses virus replication at 10 µM. SARS-CoV-2 nucleocapsid (pink; α-SARS-CoV-2 nucleocapsid), SARS-CoV-2 spike (green; α-SARS-CoV-2 S) and nuclei (DAPI) were detected. Z stacks are shown, scale bars 10 µm. Per condition and viral target, two independent transwells were processed, one stained for SARS-CoV-2 spike and one for SARS-CoV-2 nucleocapsid; representative fields of view are shown. Source data are provided as a Source Data file.

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