Fig. 5: Structural basis for HipTLp recognition by HipSLp. | Nature Communications

Fig. 5: Structural basis for HipTLp recognition by HipSLp.

From: Molecular mechanism of toxin neutralization in the HipBST toxin-antitoxin system of Legionella pneumophila

Fig. 5

a Cartoon representation of the HipTLp–HipSLp complex. HipTLp and HipSLp are colored cyan and green, respectively, and their N- and C-termini are labeled correspondingly. b Relative locations of three interacting regions formed between HipTLp and HipSLp. The two proteins are color-coded as in panel A, and HipTLp is shown in surface representation. Interacting residues of HipSLp are shown as sticks and the three regions involved in interaction with HipSLp are encircled with black, blue, and red rectangles, respectively. c–e Detailed view of the three interacting regions formed between HipTLp and HipSLp. Interacting residues are shown as sticks and hydrogen bonds are indicated with black dashed lines. f Pull-down assays performed with wild-type or mutant HipTLp carrying C-terminal 6×His-tag and untagged wild-type HipSLp.

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