Fig. 1: Mouse model of lung adenocarcinoma and identification of adenovirus-infected cell types.

a Schematic of the Eml4-Alk mouse LUAD model used. Mice were intratracheally instilled with Ad-EA and μCT was used to monitor tumour development. Representative H&E staining of an early lesion (hyperplasia) and a tumour are shown on the right. b Immunofluorescent staining of SPC (green) and CCSP (red) antibodies showing that Club cells in the bronchi start expressing SPC upon Eml4-Alk rearrangement. DAPI staining in blue. Original (left), magnified overlay (middle), and their single-channel images (right) from the dashed areas are shown. Arrowheads show cells that are double-positive CCSP⁺SPC⁺. Scale bar: 20 μm. A minimum of 6 independent animals were analysed. c Immunohistochemistry of CCSP and SPC antibodies from Eml4-Alk tumours showing CCSP⁺ bronchi as well as cells inside the tumours. Scale bars: 200 μm (left panel) and 100 μm (right panel). A minimum of 6 independent animals were analysed. d Experimental schematic of mT/mG mice transduced with Ad-Cre indicating the cell types that can get infected. e Immunofluorescent staining of the indicated antibodies on lung sections from mT/mG mice transduced with Ad-Cre, showing that Club, Ciliated, BASCs, AT2 and AT1 cells are infected. Scale bars 10 μm. A minimum of 6 independent animals were analysed.