Fig. 2: Lineage-tracing models.
From: Club cells employ regeneration mechanisms during lung tumorigenesis
a Immunofluorescent staining of GFP and the indicated antibodies in the different lineage-tracing mouse models (Scgb1a1, Sftpc, Hopx and Foxj1) showing the specific labelling (CCSP, SPC, PDPN and acTUB). Images of single-channel and overlay are displayed sequentially. Scale bars: 10 μm. A minimum of 6 independent animals were analysed. b Immunofluorescent staining of GFP and the indicated antibodies (CCSP, SPC and acTUB) in the lung sections from lineage-tracing mouse models showing the labelling specificity. Arrows indicate the unspecific labelling of the cells. Scale bars: 10 μm. A minimum of 6 independent animals were analysed. c Schematic of the labelling and tumour induction of lineage-tracing mice. d Percentage of GFP+ tumours in the respective lineage tracing mice. Scgb1a1, 3 mice and 141 tumours analysed; Sftpc, 6 mice and 214 tumours analysed; Hopx, 3 mice and 117 tumours analysed and Foxj1 4 mice and 113 tumours analysed. One-way ANOVA, Tukey’s multiple comparison test. Data are presented as mean values +/− SD. e Immunofluorescent staining of GFP and RFP antibodies in the respective mouse models. The percentages on the upper right corners represent the number of green labelled tumours out of the total number of tumours analysed in each line. Scale bars: 500 μm. Insert in the Foxj1 example is provided to show the labelling of ciliated cells in the bronchi. A minimum of 6 independent animals were analysed.
