Fig. 3: Characterization of Krt5 and Hopx mice.
From: Club cells employ regeneration mechanisms during lung tumorigenesis
a Immunofluorescent staining of GFP and KRT5 antibodies in the trachea of Krt5 mice showing labelled Basal cells. Overlay and single-channel images are sequentially presented. Scale bars: 10 μm. A minimum of 6 independent animals were analysed. b Immunofluorescent staining of GFP and the indicated antibodies in the distal lung of Krt5 mice. AT1 cells, but not Club or AT2, are labelled. Blue (DAPI), green (GFP), red (as indicated). Scale bars: 10 μm. A minimum of 6 independent animals were analysed. c Overview of GFP immunofluorescent staining in Krt5 mice. The percentage on the lower left corner represents the percentage of GFP+ tumours, 0 out of 130 tumours analysed in a total of 4 animals. Scale bars: 1 mm. A minimum of 6 independent animals were analysed. d, e GFP, SPC, and CCSP immunofluorescent staining on Hopx mice transduced with Ad-EA. Both early (d) and late (e) stages of tumorigenesis are shown; the original, magnified and single-channel images are sequentially shown from left to right; arrowheads indicate the labelled Club cells under lineage switch into AT2 cells. Scale bars: 10 μm. A minimum of 6 independent animals were analysed.
