Fig. 3: Visualising RE across densities for larger images.
a Representative image of Stx1 and Munc18-1 in primary hippocampal neurons acquired with dSTORM. Molecular localizations visualized by gaussian representation. Scale bar is 4 µm. b Zoom on single varicosity from (a), with both Gaussian representation (left) and individual localizations (right). Scale bar is 400 nm. c Voronoï-regions for tessellation of either Munc18-1 (left) or Stx1 (right). Individual localizations of opposite species are plotted beneath. Same scale as (b). d Munc18-1 regions binned by nearest neighbour distance, with mean Stx1 RE value for each bin as line plot. RE score on left-hand y-axis, and relative bin distribution on right-hand y-axis. Shaded area and black bars indicate S.E.M., n = 8 images. e Same as (d), but with reference and primary species reversed. f Localizations of the reference species color-coded by RE score for Munc18-1 (left) and Stx1 (right). g Representative axons of hippocampal neurons after treatment with NMDA (top) or TTX (bottom). h Relative enrichment of Munc18-1 across Stx1 densities after NMDA (dashed, n = 7 images) or TTX (dotted, n = 8 images) treatment. Shaded area indicates S.E.M. Inset: Mean RE of regions with an NND ≤ 10 nm for NMDA, TTX or untreated cultures (Untreat:NMDA., p = 0.004; Untreat:TTX, p = 0.084; NMDA:TTX, p = 0.039. Unpaired, two-tailed student’s t-test by image, FWER correction with Bonferroni-Holm). i Images from (g), with Stx1 localizations color-coded by RE of Munc18-1. j Spearman (S) and Mander’s (M) coefficient computed as per12 (H0: NMDA = TTX. SStx, p = 1.00; SMunc, p = 0.78; MStx, p = 1.00; MMunc, p = 0.12. Unpaired, two-tailed student’s t-test by image, FWER correction with Bonferroni-Holm). Source data are provided as a Source Data file.
