Fig. 4: The modulation of RB–E2F protein complex by CDK4/6i is responsible for enhanced viral genome replication. | Nature Communications

Fig. 4: The modulation of RB–E2F protein complex by CDK4/6i is responsible for enhanced viral genome replication.

From: Targeting the Retinoblastoma/E2F repressive complex by CDK4/6 inhibitors amplifies oncolytic potency of an oncolytic adenovirus

Fig. 4

T24 cells were pre-treated for 24 h with 0.5 µM Palbociclib and infected with ADWT or XVir-N-31 (MOI 50). a, b Cellular gene expression was analyzed by qRT-PCR at indicated time points for the genes E2F1 (a) and RB (b). Data are presented as relative mRNA expression compared to the housekeeping gene beta-actin (n = 3, mean ± SD). c Protein expression was analyzed at indicated time points by western blotting. One representative blot is shown in three independent experiments. d, e Viral gene expression was analyzed in ADWT-infected cells by qRT-PCR at indicated time points for the E1A13S gene (d) and E2-early transcripts (e). The data shown represent the mRNA expression in treated samples relative to Actin at each time point (n = 3, mean ± SD). SD standard deviation, MOI multiplicity of infection. The statistical significance was determined by a two-sided Student’s t-test; n number of biologically independent samples. Source data are provided as a Source Data file.

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