Fig. 1: Temperature-induced hydrogel formation of His-NT2RepCT and its different domains.

a Schematic presentation of the different spidroin constructs investigated herein. b Gelation times of the different recombinant spidroins (300 mg/ml) at 37 °C as tested by vial inversion. CT gelled immediately without incubation (< 300 mg/ml) and 2Rep precipitated (300 mg/ml, scale bar is 5 mm). c Time until gelation of His-NT2RepCT and NT at the indicated protein concentrations at 37 °C. d Photographs of His-NT2RepCT and NT hydrogels with a printed spider and letters “NT” underneath, respectively (both 200 mg/ml, scale bar is 5 mm).