Fig. 3: Metabolic index by integrating glucose-derived lipogenesis and FA uptake directly correlates with cisplatin resistance.

a A linear regression of glucose-d₇ intensity to IC₅₀s of cisplatin in various OC cell lines. b A linear regression of PA-d₃₁ intensity to IC₅₀s of cisplatin in various OC cell lines. c A linear regression of the ratio of PA-d₃₁/(PA-d₃₁ + Glucose-d₇) to IC₅₀s of cisplatin in various OC cell lines. n = 6 technical replicates for (a–c). d Representative bright field images, raw SRS images, and processed SRS images of ODYA and glucose-d₇ in OVCAR5 and -cisR cells. e A linear regression of the metabolic index, as defined by the ratio of C ≡ C/(C ≡ C + C-D) to IC₅₀s of cisplatin in various OC cell line pairs (COV362 (n = 8), PEO (n = 6 and 7) and OVCAR5 (n = 4)). n represents technical replicates. R² = 0.9235. Data is shown as mean ± SEM. f Representative bright field images, raw SRS images, and processed SRS images of ODYA and glucose-d₇ in primary OC cells from cisplatin treatment-resistant or sensitive patient. g Quantitative analysis of metabolic index (the ratio of C ≡ C/(C ≡ C + C-D) for primary OC cells from cisplatin treatment-resistant or sensitive patient. Each data point represents the average metabolic index of individual cancer cells from a patient and its error bar indicates the SEM; n = 30, 31, 19, 25, 27, 33, 12, 11, 24, 20 and 30 cells. The box plot indicates the analysis for each group (sensitive (n = 7 biological replicates) vs. resistant (n = 4 biological replicates)). The bound of outer box, inner box, lines, whiskers, circles represent SEM, mean, medium, 25% to 75% of data, maxima and minima, respectively. Statistical significance used two-sided Student’s t test; P = 0.011. *P < 0.05. Scale bar: 20 µm. Source data are provided in the Source Data file.