Fig. 3: Modulation of periportal hepatocytes on the metabolic microenvironment sensed by pericentral hepatocytes. | Nature Communications

Fig. 3: Modulation of periportal hepatocytes on the metabolic microenvironment sensed by pericentral hepatocytes.

From: Knowledge-graph-based cell-cell communication inference for spatially resolved transcriptomic data with SpaTalk

Fig. 3

a Slide-seq spot-based ST dataset of the mouse liver involving 25,595 spots and 17,545 genes. b Cell-type decomposition by SpaTalk. PC, pericentral; PP, periportal; Hep, hepatocytes. c Scaled Pearson’s correlation coefficients between the expression of known marker genes and the percent for each cell type. Endo, endothelial cells; DC, dendritic cells; Macro, macrophages. d Enriched LRIs that mediate cell–cell communications between pericentral and periportal hepatocytes. e Significant differentially expressed genes (DEGs) between periportal and pericentral hepatocytes assessed with the Wilcoxon test and the corresponding significantly enriched biological processes and pathways determined with the Metascape web tool. Representative DEGs are labeled beside the heatmap. f Significantly activated pathways in pericentral (up) and periportal (down) hepatocytes determined by Gene Set Enrichment Analysis (GSEA). g Communications from periportal hepatocytes to pericentral hepatocytes mediated by the Apob-Cd36 interaction in space. h Intracellular signal pathway inferred by SpaTalk over Slide-seq data and the significantly enriched pathways over the ligand–receptor–target network. i Inferred module score of each hepatocyte type over the pathway signatures determined with Seurat.

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