Fig. 1: CgMLST-based neighbor-joining tree comprising 1786 Mycobacterium abscessus isolates.

1. Strains that were previously classified into one of seven dominant circulating clones (DCC) based on core genome single nucleotide polymorphism (cgSNP)/FastBAPS analysis26. DCC clades (inner circle) are delineated and colored based on the most recent common ancestral node comprising all strains with known DCC classification. 2. Sequence types (ST) according to the nomenclature of the 7-loci MLST scheme hosted on pubMLST47. 3. Mash distance-based subspecies classification.