Fig. 8: Optogenetic silencing of ACC neurons during outcome feedback period following animals’ 2nd choices.
From: Cingulate-motor circuits update rule representations for sequential choice decisions
a Histological section for halorhodopsin (eNpHR3.0) expression in ACC. Green, eNpHR3.0-eYFP expression. Blue, DAPI. White dotted line shows reconstructed positions of fiberoptic implants. Scale bar, 500 μm. b Suppression of spiking activity by 561 nm light delivery. Top left, raster plot of a representative single-unit measured in ACC showing spiking activities before, during, and after laser light delivery. Top right, example waveforms of the representative single-unit. Bottom left, peri-event time histogram sorted by the timing of light onset. Bin width, 50 ms. c Optogenetic silencing of ACC after animal’s incorrect 2nd choices (i.e., 2nd choice omission errors). Light was delivered for 4 s after animals pushing the center lever instead of correctly poking the side port opposite to the 1st choice. d Optogenetic silencing of ACC after animal’s correct 2nd choices. Light was delivered for 4 s after a correct 2nd choice (i.e., animals poking the side port opposite to the 1st choice). Source data are provided as a Source Data file.
