Fig. 2: On-target editing of AID-2S, −3S and rAPOBEC1 base editors in HEK293T.

a On-target editing profiles of the CBE variants analyzed by deep sequencing at eight sites in HEK293T. The sequences of the eight target sites are aligned and the nucleotide positions are numbered from the next to the 5′ end of the PAM sequence. The editing efficiency at each cytosine base position were averaged across all sites. Data are presented as mean values ± sd (n = 8). The mutation frequencies for each target sites are also shown in Supplementary Fig 6. b Domain architectures of SaAID, SaAID-2S and SaAID-3S. The gRNA expression cassette was combined into each effector plasmid. c On-target editing frequencies at the peak base position are shown for of SaAID, SaAID-2S and SaAID-3S, and data are presented as mean values ± sd (n = 3). To normalize transfection efficiencies, cells were sorted by the expression of iRFP670 from the plasmid backbone. The mutation frequencies of each nucleotide position are also shown in Supplementary Fig. 11. Source data are provided as a Source Data file.