Fig. 4: Dietary supplementation of CS, not cholesterol, alleviates colitis in mice. | Nature Communications

Fig. 4: Dietary supplementation of CS, not cholesterol, alleviates colitis in mice.

From: Cholesterol sulfate alleviates ulcerative colitis by promoting cholesterol biosynthesis in colonic epithelial cells

Fig. 4

a Schematic diagram of the treatment and body weight loss rate of C57BL/6J, low dose cholesterol-fed (LCHOL-fed) C57BL/6J, and CS-fed C57BL/6J mice during the DSS challenge (n = 4 mice/group, LCHOL: low dose (0.005%) cholesterol). Two-way ANOVA with Tukey’s multiple comparisons test, day 5 (DSS vs. DSS + CS) **P = 0.0028; day 6 (DSS vs. DSS + CS) ***P < 0.0001. b Qualification of CS in cecal contents and colonic tissues from C57BL/6J and CS-fed C57BL/6J mice using LC–MS (n = 5 mice/group, two-tailed Student’s t-test). c FOB and colon length/body weight of C57BL/6J and CS-fed C57BL/6J mice (n = 5 mice/group, two-tailed Student’s t test). d Schematic diagram of the treatment and body weight loss rate of C57BL/6 mice challenged with 33-day 1% DSS and fed with or without 0.004% CS (n = 6 mice/group), two-way ANOVA with Tukey’s multiple comparisons test, day 27 (DSS vs. DSS + CS) *P = 0.0485; day 33 (DSS vs. DSS + CS) *P = 0.0319. e FOB of C57BL/6J mice challenged with 33-day 1% DSS and fed with or without 0.004% CS on the 9th, 18th, and 27th day (n = 6 mice/group, two-tailed Student’s t-test). f Schematic diagram of the treatment and survival proportion of C57BL/6J mice challenged with 3-round 2.5% DSS and fed with or without 0.004% CS (Gehan-Breslow-Wilcoxon Test). g FOB of C57BL/6J mice challenged with 3-round 2.5% DSS and fed with or without 0.004% CS on the 5th (n = 7, 6 mice/group, respectively) and 15th (n = 7, 4 mice/group, respectively, two-tailed Student’s t-test). h Schematic diagram of the treatment and body weight increase rate of interleukin-10 deficient (IL-10−/−) mice fed with (n = 7) or without (n = 6) 0.004% CS. Two-way ANOVA with Tukey’s multiple comparisons test, day 37 (IL-10−/−Control vs. IL-10−/−CS) *P = 0.0338; day 42 (IL-10−/−Control vs. IL-10−/−CS) *P = 0.0437. i FOB of interleukin-10 deficient (IL-10−/−) mice fed with (n = 6) or without (n = 6) 0.004% CS on the 14th day and 35th day. (Two-tailed Student’s t-test). j Colon length/body weight of interleukin-10 deficient (IL-10−/−) mice fed with (n = 7) or without (n = 6) 0.004% CS. (Two-tailed Student’s t-test). Data from in vitro assays are reprehensive of at least three independent experiments. Data are shown as the mean ± SEM. Source data are provided as a Source Data file.

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