Fig. 7: SREBP2 inhibitor betulin suppresses the alleviation effects of CS on DSS-induced colitis in mice. | Nature Communications

Fig. 7: SREBP2 inhibitor betulin suppresses the alleviation effects of CS on DSS-induced colitis in mice.

From: Cholesterol sulfate alleviates ulcerative colitis by promoting cholesterol biosynthesis in colonic epithelial cells

Fig. 7

a, b Total and free cholesterol content of colonic tissues from C57BL/6J mice in the presence or absence of CS (0.004%). (n = 5 mice/group, two-tailed Student’s t-test). c Schematic diagram of treatment and body weight reduction rate in C57BL/6J, 0.004% CS-fed C57BL/6J, C57BL/6J + betulin (100 mg/kg), and 0.004% CS-fed C57BL/6J + betulin (100 mg/kg) mice during DSS challenge (n = 5 mice/group). Two-way ANOVA with Tukey’s multiple comparisons test, day 5 (DSS + CS vs. DSS + CS + Betulin) **P = 0.0065; day 6 (DSS + CS vs. DSS + CS + Betulin) ***P < 0.0001. Data are shown as the mean ± SEM. d, e FOB and colon length/body weight of these mice (n = 5 mice/group, one-way ANOVA with Sidak’s multiple comparison test). f, g Hematoxylin and eosin and EdU staining of colonic sections excised from the same parts (2 cm away from the anus) of these mice (n = 5 mice/group, one-way ANOVA with Sidak’s multiple comparison test). h Schematic presentation of the role of CS in intestinal epithelial repairment in colitis. Data are shown as the mean ± SEM. Source data are provided as a Source Data file.

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