Fig. 1: Hepatic deletion of Wtap causes non-alcoholic steatohepatitis (NASH) under the normal chow feeding condition.
From: Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH)
a WTAP protein levels in liver, iBAT, iWAT, eWAT and skeletal muscle of WT mice. The samples were derived from the same experiment and the blots were processed in parallel. This experiment was repeated for three times independently with similar results. b WTAP protein levels in liver, iBAT, skeletal muscle and eWAT of Wtapflox/flox and Wtap-HKO mice at 8 weeks old. The samples were derived from the same experiment and the blots were processed in parallel. This experiment was repeated for three times independently with similar results. c Body weights of Wtapflox/flox and Wtap-HKO mice at 8 weeks old under the normal chow feeding condition (Wtapflox/flox, n = 10; Wtap-HKO, n = 8; P = 0.0057). d Representative pictures of livers from Wtapflox/flox and Wtap-HKO mice. e Liver weights (Wtapflox/flox, n = 10; Wtap-HKO, n = 8; P = 0.0011). f Liver TAG levels (n = 8 for each group; P < 0.0001). g Oil Red O staining was performed in the liver sections from Wtapflox/flox and Wtap-HKO mice at 8 weeks old (n = 5 for each group). n was the number of biologically independent mice. Mice of the same genotype showed similar phenotypes. Representative Oil Red O staining images were shown. h Serum ALT activity in Wtapflox/flox and Wtap-HKO mice at 8 weeks old (n = 8 for each group; P < 0.0001). i The TUNEL-positive cells in liver sections of Wtapflox/flox and Wtap-HKO mice at 8 weeks old (n = 6 for each group; P < 0.0001). j Cleaved caspase3 levels were measured by immunoblotting (n = 4 for each group). The samples were derived from the same experiment and the blots were processed in parallel. This experiments were repeated for three times with similar results. k F4/80 immunostaining of liver sections (n = 5 for each group; P < 0.0001). l Sirius Red staining of liver sections (n = 5 for each group; P = 0.0006). m RT-qPCR analysis of mRNA levels (n = 8 for each group; Collagen IA1, P = 0.0063; αSma, P = 0.0234; Mmp9, P = 0.0006; Tgfb1, P = 0.0003). n was the number of biologically independent mice. Data represent the mean ± SEM. Significance was determined by unpaired two-tailed Student’s t test analysis. *p < 0.05. **p < 0.01. Source data are provided as a Source Data file.
