Fig. 3: Liver-specific knockout of Wtap promotes lipolysis in eWAT.
From: Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH)
a Serum FFA levels in Wtapflox/flox and Wtap-HKO mice at 8 weeks old (Wtapflox/flox, n = 12; Wtap-HKO, n = 11; P = 0.0028). b Relative serum palmitate, linoleate, myristate, stearate and arachidonate levels (n = 5 for each group; palmitate, P = 0.0028; linoleate, P = 0.0019; myristate, P = 0.0026; stearate, P < 0.0001; arachidonate, P = 0.08377). c Serum palmitic acid levels (n = 5 for each group; P = 0.0026). d The relative eWAT weights in Wtapflox/flox and Wtap-HKO mice at 8 weeks old (n = 8 for each group; P = 0.0003). e p-HSL, HSL, p-PKA substrate, ATGL and GAPDH protein levels were measured by immunoblotting in the eWAT of Wtapflox/flox and Wtap-HKO mice (n = 4 for each group). p-HSL, p-PKA substrate and ATGL protein levels were quantified by Image J (n = 4 for each group; p-HSL/HSL, P = 0.00077; ATGL/GAPDH, P = 0.039; p-PKA substrate/GAPDH, P = 0.03358). The samples were derived from the same experiment and the blots were processed in parallel. f cAMP levels in eWAT of Wtapflox/flox and Wtap-HKO mice at 8 weeks old (n = 11 for each group; P = 0.0044). g RT-qPCR analysis of Adcys mRNA levels in eWAT of Wtapflox/flox and Wtap-HKO mice at 8 weeks old (Wtapflox/flox, n = 11; Wtap-HKO, n = 8; Adcy1, P = 0.6368; Adcy2, P = 0.2823; Adcy3, P = 0.00068; Adcy4, P = 0.00028; Adcy5, P = 0.1098; Adcy6, P = 0.0254; Adcy7, P = 0.6631; Adcy8, P = 0.2374; Adcy9, P = 0.8768). h ADCY3, ADCY4 and ADCY6 protein levels in eWAT of Wtapflox/flox and Wtap-HKO mice at 8 weeks old (n = 4 for each group). The samples were derived from the same experiment and the blots were processed in parallel. i Ex vivo lipolysis assay (Glycerol release) in the eWAT treated with or without isoproterenol (120 nM) for 4 hours (n = 3 for each group; Basal, P < 0.0001; Iso, P = 0.0085). This experiment was repeated for three times independently. n was the number of biologically independent mice. Data represent the mean ± SEM. Significance was determined by unpaired two-tailed Student’s t test analysis. *p < 0.05. **p < 0.01. Source data are provided as a Source Data file.
