Fig. 2: KIR2DL1 transgene recognizes HLA-C*05, modifies NK cell function. | Nature Communications

Fig. 2: KIR2DL1 transgene recognizes HLA-C*05, modifies NK cell function.

From: Mouse fetal growth restriction through parental and fetal immune gene variation and intercellular communications cascade

Fig. 2

a Experimental design of NK cell stimulation experiments. b Staining for IFNγ+ NK cells (CD3-TCR– Nkp46+ or CD3-TCR-DX5+) upon culture with antibodies depicted in panel a. c Experimental design of adoptive transfer experiments. d Representative staining of cellular injection mix and CFSE+ splenocytes harvested from recipient mice. Numbers denote percentage of each gated cell population. e Relative survival of HLA-C*05+ KO cells compared to survival of HLA-C*05-KO cells (normalized to WT mice). f Representative flow cytometric staining of DX5 and CD49a on uNK cells (CD3-CD19-TCR-CD45+NKp46+CD122+ cells) isolated from the gd9.5 implantation sites from the mentioned mating crosses. KIR staining is shown on total uNK cells, CD49a+, or DX5+ uNK subsets. g Percentage of KIR+ uNK cells in different mating crosses at gd9.5. Crosses are written as female × male. h HLA-C*05 mRNA expression on fetal trophoblast cells from gd9.5 or gd10.5 implantation sites. i Representative HLA-C*05 cell surface expression on fetal trophoblast cells isolated from placenta at gd12.5, and cultured with (solid lines) or without stimulation (dashed lines). Purple: HLA-C*05+ trophoblast cells, gray: HLA-C*05– trophoblast, i.e., WT cells, filled histogram: fluorescence minus one (FMO) control. Numbers denote MFI. j HLA-C cell surface expression plotted as MEF normalized to FMO controls. Mean ± SEM is shown. n represents biologically independent animals/samples in each group. b n = 3 (WT), 10 (HLA-C*05), 4 (KIR2DL1) for α-NKp46 ± α-KIR stim, n = 4 (WT), 9 (HLA-C*05), 4 (KIR2DL1) for α-Ly49D ± α-KIR stim. e n = 9 (WT), 6 (KIR2DL1). g n = 10 (WT × C*05), 9 (KIR × C*05) and 8 (C*05/KIR × WT). h n = 4 per group. j n = 5 per group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, Two-tailed Mann–Whitney U-test. Exact p-values are: b p = 0.0286 (both comparisons), e p = 0.0002, g p < 0.0001, h p = 0.0286, j p = 0.0079 (both comparisons). Source Data are provided as a Source Data file.

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