Fig. 1: D. melanogaster oviposition rates are altered in the presence of young Lb females.

a Standard oviposition assay design. Each bottle contained twenty Canton-S (CS) female flies and five CS male flies, either with twenty female Lb wasps (exposed) or with no wasps (unexposed). Flies aged 3 days post-eclosion and wasps aged 2 days post-emergence were used. The food dishes were replaced daily, and the eggs laid each day were counted. b The daily number of eggs laid by the unexposed and exposed CS flies. Flies were exposed to wasps for 20 days. The experiment was performed eighteen times. Data represent the mean ± SEM. Significance was determined by two-way ANOVA with Sidak’s multiple comparisons test, p values are indicated in Source Data file (***p < 0.001; ns, not significant). c The daily number of eggs laid by the unexposed flies and flies in bottles with 2-day-old female wasps at the beginning that were replaced by 4-day-old females on Day 7 (exposed). The experiment was performed nine times. Data represent the mean ± SEM. Significance was determined by two-way ANOVA with Sidak’s multiple comparisons test, p values are indicated in Source Data file (***p < 0.001; ns, not significant). d The daily number of eggs laid by the unexposed flies and flies in bottles with 2-day-old female wasps at the beginning that were replaced by 12-day-old females on Day 7 (exposed). The experiment was performed nine times. Data represent the mean ± SEM. Significance was determined by two-way ANOVA with Sidak’s multiple comparisons test, p values are indicated in Source Data file (**p < 0.01; ***p < 0.001; ns, not significant). Source data are provided as a Source Data file.