Fig. 4: Decreased oviposition depends on visual cues. | Nature Communications

Fig. 4: Decreased oviposition depends on visual cues.

From: Search performance and octopamine neuronal signaling mediate parasitoid induced changes in Drosophila oviposition behavior

Fig. 4

a The daily number of eggs of blind GMR-grim flies exposed to Lb female wasps compared to unexposed flies. Blind flies were exposed to wasps for a testing period of 10 days. The experiment was performed ten times. Data represent the mean ± SEM. Significance was determined by two-way ANOVA with Sidak’s multiple comparisons test, p values are indicated in Source Data file (ns, not significant). b The daily number of eggs of olfactory-deficient Orco1 mutant flies exposed to Lb female wasps compared to that of unexposed flies. Olfactory-deficient flies were exposed to wasps for a testing period of 10 days. The experiment was performed eleven times. Data represent the mean ± SEM. Significance was determined by two-way ANOVA with Sidak’s multiple comparisons test, p values are indicated in Source Data file (***p < 0.001; ns, not significant). c Oviposition experiment setup for the dark conditions. d Egg numbers of CS flies exposed to 4-day-old (young) or 12-day-old (old) Lb females compared with unexposed flies under dark conditions. Eggs were counted on Day 2. The experiment was performed eight times. Data represent the mean ± SEM. Significance was determined by two-way ANOVA with Sidak’s multiple comparisons test, p values are indicated in Source Data file (ns, not significant). e Experimental setup for visual exposure only of flies and wasps. f Egg numbers of CS flies only visually exposed to 4-day-old (young) or 12-day-old (old) Lb females compared with unexposed flies. Eggs were counted on Day 2. The experiment was performed at least eight times. Data represent the mean ± SEM. Significance was determined by two-way ANOVA with Sidak’s multiple comparisons test, p values are indicated in Source Data file (***p < 0.001; ns, not significant). g The number of eggs laid by the unexposed and exposed D. melanogaster females, including UAS-TNT, LC4-GAL4, and LC4-GAL4 > UAS-TNT genotypes. Eggs were counted on Day 2. The experiment was performed nine times. Data represent the mean ± SEM. Significance was determined by two-way ANOVA with Sidak’s multiple comparisons test, p values are indicated in Source Data file (***p < 0.001; ns, not significant). Source data are provided as a Source Data file.

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