Fig. 5: Exposure to wasps decreases follicle rupture and Mmp2 activity. | Nature Communications

Fig. 5: Exposure to wasps decreases follicle rupture and Mmp2 activity.

From: Search performance and octopamine neuronal signaling mediate parasitoid induced changes in Drosophila oviposition behavior

Fig. 5

a Images of mature eggs (red arrows) from single pairs of ovaries of the unexposed and exposed Drosophila on Day 2 and Day 10. Scale bars, 400 μm. b The number of mature eggs per ovary in the unexposed and exposed flies on Day 2 and Day 10. n = 50 per group. Data represent the mean ± SEM. Significance was determined by two-way ANOVA with Sidak’s multiple comparisons test, p values are indicated in Source Data file (***p < 0.001; ns, not significant). c Images of DAPI-stained mature eggs with partial follicle rupture (red arrow) and oocytes fully covered by follicular cells. Six biologically independent experiments were performed. Scale bars, 100 μm. d Percent of mature eggs with follicle rupture of the unexposed and exposed flies on Day 2 and Day 10. n = 6 per group; ~30 mature oocytes per replicate. Data represent the mean ± SEM. Significance was determined by two-way ANOVA with Sidak’s multiple comparisons test, p values are indicated in Source Data file (***p < 0.001; ns, not significant). e In situ zymography images of Mmp2 activity (green) in mature eggs of ovaries of the exposed and unexposed flies collected on Day 2 and Day 10. Egg chambers with posterior Mmp2 activity are marked by white arrowheads. Scale bars, 400 μm. f Percent of eggs with Mmp2 activity of the unexposed and exposed flies on Day 2 and Day 10. n = 6 per group; ~30 mature oocytes per replicate. Data represent the mean ± SEM. Significance was determined by two-way ANOVA with Sidak’s multiple comparisons test, p values are indicated in Source Data file (***p < 0.001; ns, not significant). g Cartoon of the Drosophila ovary and oviduct. The focus region in the upper common oviduct (dashed box) is indicated. h Representative images of single myofibrils from oviducts of the unexposed and exposed groups. The distance between each GFP band represents the sarcomere length. Three biologically independent experiments were performed. Scale bars, 2 μm. i Sarcomere lengths in focus regions of oviducts of the unexposed and exposed females on Day 2 and Day 10. n = 30 per group. Data represent the mean ± SEM. Significance was determined by two-way ANOVA with Sidak’s multiple comparisons test, p values are indicated in Source Data file (***p < 0.001; ns, not significant). Source data are provided as a Source Data file.

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