Fig. 6: Exposure to wasps decreases OA production in VNC. | Nature Communications

Fig. 6: Exposure to wasps decreases OA production in VNC.

From: Search performance and octopamine neuronal signaling mediate parasitoid induced changes in Drosophila oviposition behavior

Fig. 6

a Cartoon of the female reproductive tract and the brain, VNC, and thoracic abdominal ganglion. Green spots represent the OA neurons. PTS, single cell in midline of the prothoracic neuromere; PTC, cell cluster in midline of the prothoracic neuromere; MSC, cell cluster in midline of the mesothoracic neuromere; MTC, cell cluster in midline of the metathoracic neuromere; AC, cell cluster in the thoracic abdominal ganglia. b Quantification of Tdc2 and Tβh mRNA levels in the brains of the unexposed and exposed female flies on Day 2. n = 4 per group. Data represent the mean ± SEM. Significance was determined by two-sided unpaired Student’s t test, p values are indicated in Source Data file (ns, not significant). c Quantification of Tdc2 and Tβh mRNA levels in the brains of the unexposed and exposed female flies on Day 10. n = 4 per group. Data represent the mean ± SEM. Significance was determined by two-sided unpaired Student’s t test, p values are indicated in Source Data file (ns, not significant). d Quantification of Tdc2 and Tβh mRNA levels in the VNC of the unexposed and exposed female flies on Day 2. n = 4 per group. Data represent the mean ± SEM. Significance was determined by two-sided unpaired Student’s t test, p values are indicated in Source Data file (**p < 0.01). e Quantification of Tdc2 and Tβh mRNA levels in the VNC of the unexposed and exposed female flies on Day 10. n = 4 per group. Data represent the mean ± SEM. Significance was determined by two-sided unpaired Student’s t test, p values are indicated in Source Data file (ns, not significant). f Representative images of OA (white) immunolocalization in the unexposed and exposed VNC of female flies on Day 2. Three biologically independent experiments were performed. Scale bars, 50 μm. g Fluorescence intensity of OA immunolocalization in the unexposed and exposed octopaminergic neurons of the indicated clusters of female flies on Day 2. Plotted is the mean intensity from 3 areas within each cell. Left to right, n = 51, 60, 56, 66, 52, 62, 84, and 96. Data represent the mean ± SEM. Significance was determined by two-way ANOVA with Sidak’s multiple comparisons test, p values are indicated in Source Data file (***p < 0.001). Source data are provided as a Source Data file.

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