Fig. 7: OA mediates parasitic wasp-induced suppression of oviposition.
a Cartoon of the female reproductive tract regions, including the ovary (OV), lateral oviduct (LO), upper common oviduct (COU), and lower common oviduct (COD). b Representative image of OA (white) immunolocalization in the unexposed and exposed COU regions of female flies on Day 2. Three biologically independent experiments were performed. Scale bars, 10 μm. c Fluorescence intensity of OA immunolocalization in the unexposed and exposed female reproductive tract regions of female flies on Day 2. Plotted is the overall mean intensity for each specific region. Left to right, n = 29, 25, 29, 32, 30, 32, 30, and 32. Data represent the mean ± SEM. Significance was determined by two-way ANOVA with Sidak’s multiple comparisons test, p values are indicated in Source Data file (***p < 0.001). d The number of eggs laid by the unexposed and exposed D. melanogaster female flies, including Tdc2-GAL4, UAS-TRPA1, and Tdc2-GAL4 > UAS-TRPA1 flies of different genotypes. Eggs were counted on Day 2. The temperature of 23 °C is nonactivating, and 29 °C increases the activity of OA neurons. The experiment was performed eight times. Data represent the mean ± SEM. Significance was determined by two-way ANOVA with Sidak’s multiple comparisons test, p values are indicated in Source Data file (***p < 0.001; ns, not significant). e The number of eggs laid by unexposed and exposed D. melanogaster female flies, including Tdc2-GAL4, UAS-eagDN, and Tdc2-GAL4 > UAS-eagDN flies of different genotypes. Eggs were counted on Day 2. Overexpression of eagDN increases OA neuronal activity. The experiment was performed eight times. Data represent the mean ± SEM. Significance was determined by two-way ANOVA with Sidak’s multiple comparisons test, p values are indicated in Source Data file (***p < 0.001). f The number of eggs laid by unexposed and exposed flies 1 day post OA or doubly distilled H2O (ddH2O) injection. The experiment was performed eight times. Data represent the mean ± SEM. Significance was determined by two-way ANOVA with Sidak’s multiple comparisons test, p values are indicated in Source Data file (***p < 0.001). Source data are provided as a Source Data file.
