Fig. 4: ACS101 light chain interactions with the CD4bs of AMC009 SOSIP and comparison to other VH1-2 and VH1-46-derived CD4bs bNAbs. | Nature Communications

Fig. 4: ACS101 light chain interactions with the CD4bs of AMC009 SOSIP and comparison to other VH1-2 and VH1-46-derived CD4bs bNAbs.

From: Identification of IOMA-class neutralizing antibodies targeting the CD4-binding site on the HIV-1 envelope glycoprotein

Fig. 4

a Interaction of ACS101, IOMA (PDB:5T3Z) and VRC01 (3NGB) with the V5 loop. All interactions are shown with the gp120 subunit that the bNAbs were complexed with: ACS101 (clade B AMC009 SOSIP), IOMA (clade A BG505.664 SOSIP) and VRC01 (clade A/E 93TH057 gp120). b Comparison of the V5 loop of ACS101 and PGV04 (PBD:6VO3) bound to gp120. Both ACS101 and PGV04 are in complex with AMC009 SOSIP. The shift of the V5 loop is indicated as a dotted line measured from Cα- Cα of residue S460. c Interactions of ACS101 with the V5 loop and the N276 glycan. d Ribbon representations Fabs (only the light chain is shown) of ACS101 and other VH1-2 and VH1-46-derived CD4bs bNAbs (liganded) with the CDRL1 colored in red. ACS101 is shown as liganded and unliganded Fab. IOMA (PDB:5T3Z), VRC01 (PDB:3NGB) and 8ANC131 (PDB:4RWY) Fabs are liganded. e Binding of ACS101, ACS101iGL CDRL1 and ACS101IOMA CDRL1 to AMC009 SOSIP and AMC009 2 M 1F9 SOSIP using biolayer interferometry (BLI). The bNAb 2G12 and PBST + BSA (PBS pH 7.4 + 0.01% (w/v) BSA + 0.002% (v/v) Tween 20) were included as a loading and negative control, respectively. f Neutralization of AMC009 by ACS101, ACS101iGL CDRL1 and ACS101IOMA CDRL1. The length of the error bars is the standard deviation at each concentration (n = 2 biologically independent experiments).

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