Fig. 3: Shigella infection upregulates SIRT2 through Golgi stress.
From: Golgi stress induces SIRT2 to counteract Shigella infection via defatty-acylation
A SIRT2 protein level is induced by IpaJ, showing by immunoblots for SIRT2 protein levels in HEK293T cells with or without Flag-IpaJ overexpression. HSP90 is the loading control. B Immunoblots of SIRT2 in BMDMs infected with mock, wildtype or IpaJ deletion S. flexneri M90T. The BMDMs were derived from 6 to 8-week old wildtype C57BL/6 J mice. Quantification of SIRT2 protein levels (normalized to actin) in the immunoblots is shown on the right. Statistical evaluation was done by student t-test. C RT-PCR of Sirt2 (left) and Creb3 (right) in BAL cells from mice intranasally infected with mock, wildtype or IpaJ deletion S. flexneri M90T cells. mRNA level in each sample was normalized to internal control (Actb). Statistical evaluation was done by one-way ANOVA. Data are represented as mean ± SEM. a.u., arbitrary unit.
