Fig. 1: Increased HSC numbers in mice with CXCR4-deficient MPPs. | Nature Communications

Fig. 1: Increased HSC numbers in mice with CXCR4-deficient MPPs.

From: Competition between hematopoietic stem and progenitor cells controls hematopoietic stem cell compartment size

Fig. 1

a Schematic representation of CXCR4 deletion in hematopoietic multipotent progenitor cells with a Flk2-cre transgene. b Gating strategy for LSKs, LT-HSC, ST-HSC, and MPP4 cells. c, d LT-HSC, ST-HSC, and MPP4 cell numbers in bone marrow (c) and in spleen (d). e LSK cell number in bone marrow. In panels C-E, cells were collected from one femur and tibia, or from spleen, of Flk2-cre.Cxcr4fl/+ (CTR, black) and Flk2-cre.Cxcr4fl/fl mice (cKO, red). f HSC chimerism in bone marrow of mice reconstituted with 50% CD45.2+ CTR or cKO bone marrow cells mixed with 50% CD45.1+ wild-type bone marrow cells. Data in all panels are representative of two or more experiments. Panels c and e are derived from n = 4 mice per group, panel d is from n = 3/group, and panel F is derived from 10 mice per group. Bars indicate average, circles depict individual mice. *P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001 by unpaired two-sided Student’s t test. Source data are provided as a Source Data file.

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