Fig. 2: Measurement of HSC quiescence and self-renewal in mice with CXCR4-deficient MPPs. | Nature Communications

Fig. 2: Measurement of HSC quiescence and self-renewal in mice with CXCR4-deficient MPPs.

From: Competition between hematopoietic stem and progenitor cells controls hematopoietic stem cell compartment size

Fig. 2

a, b HSC cell cycle status. a Gating strategy and cell frequency (n = 4 mice/group); (b) Enumeration of HSCs in G0 (Ki67-) and in G1/S/G2M (Ki67+) in bone marrow (n = 4 mice/group). c Kaplan–Meyer survival plot of mice following weekly 5-FU treatment (i.p., 150 mg/kg, n = 8 per group). a–c data obtained from Flk2-cre.Cxcr4fl/+ (black) and Flk2-cre.Cxcr4fl/fl (red) mice (d) Serial transplantation: Ratio of bone marrow chimerism in HSCs displayed as chimerism in secondary transplantation divided by chimerism in primary transplantation. Mice were reconstituted with 50% CD45.2+ CTR or cKO bone marrow cells mixed with 50% CD45.1+ wild-type bone marrow cells (n = 10/group). e–g Long-term self-renewal and differentiation potential of 30 phenotypic HSCs analyzed during primary and secondary transplantation (n = 5 and n = 8). e HSC chimerism; (f) CD19+ B cell chimerism; (g) Gr1+ granulocyte chimerism. Data in all panels are representative of two or more experiments. Bars and lines indicate average, circles depict individual mice. n.s. not significant; P > 0.05 by unpaired two-sided Student’s t test. Source data are provided as a Source Data file.

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