Fig. 6: Downregulation of the dcw cluster in N. elongata ΔmraZ.

a Volcano plot of RNAseq analysis of an N. elongata ΔmraZ and complemented. p value is plotted against fold change and were calculated using DeSeq2. Red points represent genes upregulated in MraZ-overexpressing N. elongata (ΔmraZ; porBp-mraZ – i.e., mraZ under the control of the strong and constitutive porB promoter), as compared to N. elongata ΔmraZ. b Venn diagram showing genes (mraZ, mraW, ftsL and ftsI) upregulated in N. elongata wild-type as compared to N. elongata ΔmraZ. c Transcript abundance of dcw cluster genes measured by qRT-PCR in N. elongata expressing or not expressing MraZ. Data represent mean (n = 3 biologically independent samples ± SD) and are representative of three independent experiments. Statistical test used was Unpaired t test with Welch’s correction by comparing ΔmraZ to the parental wild-type and the ΔmraZ; porBp-mraZ to the parental ΔmraZ (ns not significant). d Scanning electron microscopy of N. elongata expressing or not expressing MraZ. Scale bar is 2 µm. e Median cell length measurements of N. elongata expressing or not expressing MraZ (n = 120 biologically independent cells). Data are presented with the median and are representative of at least two independent experiments. Statistical test used was One-way ANOVA, with Bonferroni’s multiple comparisons test (***p < 0.001). Source data and statistics are provided as a Source Data file (for a, c and e).