Fig. 7: IRX-type leukemia-initiating potential is lost earlier in B-cell development than HOXA-type potential. | Nature Communications

Fig. 7: IRX-type leukemia-initiating potential is lost earlier in B-cell development than HOXA-type potential.

From: Multi-omics analysis defines highly refractory RAS burdened immature subgroup of infant acute lymphoblastic leukemia

Fig. 7

a Schematic outline of in vitro CB transformation assay. CB HSPCs were sorted, transduced with KMT2A-Aff1, and cultured on MS-5 stroma cells under lymphoid conditions. b Flow cytometry plots of KMT2A-Aff1-transformed HSPCs. c RT-PCR of IRX1 and HOXA9 in the transformed HSPCs. HOXA-type (PER-494) and IRX-type (PER-785) cell lines are shown as controls. Experiments were independently repeated three times, and representative results are shown. Source data are provided as a Source Data file.

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