Table 2 purification of ferredoxin-NAD+ reductases from C. acetobutylicum

From: Molecular characterization of the missing electron pathways for butanol synthesis in Clostridium acetobutylicum

Steps

Eluted peak

Activity (units)

Protein (mg)

Specific activity (units/mg)

Recovery (%)

Purification fold

Crude extract

 

13.4

203

0.07

100

1

Streptomycin sulfate supernatant

 

9.02

102

0.09

67

1.28

Capto-DEAE column

Major peak 1

7.3

5.44

1.34

54

19.14

Minor peak 2

1.27

4,06

0.31

9.5

4.49

Peak 1 on Superose 12 column

 

3.43

1.5

2.29

25

32.7

Peak 2 on Resource Q column

 

0.56

1.5

0.40

4.2

5.71

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