Fig. 3: Proteolytic processing is not necessary for in vitro activity of PM X. | Nature Communications

Fig. 3: Proteolytic processing is not necessary for in vitro activity of PM X.

From: Maturation and substrate processing topography of the Plasmodium falciparum invasion/egress protease plasmepsin X

Fig. 3

Second copy PM X-GFP was purified from synchronized 42–45 h schizonts using anti-GFP antibody. The pulled down proteins were then incubated with fluorogenic Rh2N substrate peptide (1 μM) at the indicated pH. In control wells, CWHM-117 (1 μM) was added to inhibit PM X activity. Reactions were carried out for 1 h at 37 °C. Mean values from three independent experiments are shown and error bars represent standard deviations. Data were analyzed statistically by two-tailed Student’s t test, p values are shown on the graph. Source data are provided as a Source Data file.

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