Fig. 7: PM X cleaves microneme substrates intracellularly, while rhoptry substrate cleavage is mediated in a post-secretion manner. | Nature Communications

Fig. 7: PM X cleaves microneme substrates intracellularly, while rhoptry substrate cleavage is mediated in a post-secretion manner.

From: Maturation and substrate processing topography of the Plasmodium falciparum invasion/egress protease plasmepsin X

Fig. 7

a, b PM Xapt parasites were grown in presence or absence of aTc to the schizont stage. At 44 h post invasion, either C1 (1.5 μM) or E64d (10 μM) was added to the +aTc cultures for 6 h before harvesting. Samples were blotted assess the cleavage of either AMA1 (a microneme substrate) or Rh5 (b rhoptry substrate). Processing of SUB1 and SERA5 were analyzed from the same lysates as indicators of PM X activity and exoneme discharge respectively. Each blot was repeated at least twice. Source data are provided as a Source Data file.

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