Fig. 8: PM X and SUB1 signals overlap significantly more with microneme markers (AMA1 and EBA175) than with rhoptry neck (RON4) or bulb (RAP1) markers in terminal schizonts. | Nature Communications

Fig. 8: PM X and SUB1 signals overlap significantly more with microneme markers (AMA1 and EBA175) than with rhoptry neck (RON4) or bulb (RAP1) markers in terminal schizonts.

From: Maturation and substrate processing topography of the Plasmodium falciparum invasion/egress protease plasmepsin X

Fig. 8

Synchronized, C1-treated 48–50 h schizonts expressing either PM X-3xHA or SUB1-3xHA were fixed in paraformaldehyde and processed for immunofluorescence assays. After labeling with the indicated antibodies, samples were visualized by confocal Airyscan microscopy. Images in a are 2D snapshots. 3D reconstructions are shown in b. Each grid line in b is 1.61 μm. Shown are representative images from two independent experiments. c Quantification of the 3D images from b. For each line, 10 schizonts were analyzed from three biological replicates. Mean values are shown and error bars represent standard deviations. Data were analyzed statistically by two-tailed Student’s t test, p values are shown on the graphs. Source data are provided as a Source Data file.

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