Fig. 9: A subset of the apically located vesicles in schizonts show colocalization between AMA1 and PM X or SUB1 by immunoEM. | Nature Communications

Fig. 9: A subset of the apically located vesicles in schizonts show colocalization between AMA1 and PM X or SUB1 by immunoEM.

From: Maturation and substrate processing topography of the Plasmodium falciparum invasion/egress protease plasmepsin X

Fig. 9

Synchronized, C1-treated, 48–50 h schizonts expressing either PM X-3xHA (a) or SUB1-3xHA (b) were processed as described in the methods. Thin section samples were labeled for the indicated markers and visualized by immunoelectron microscopy. Black arrows: 18 nm beads that detect the anti HA antibody, yellow arrows: 12 nm beads that detect the anti AMA1 antibody. Sections showing apical vesicles are magnified from each image. R rhoptry, N nucleus. Shown are representative images from two independent experiments.

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