Fig. 3: Characterizing mutations in the promoter region of PP_2569 and XylE.

a Heatmap of the selected intracellular and extracellular metabolites. Z-scores were calculated using the average intensities of intracellular and extracellular metabolites separately, and a time zero control from uninoculated medium was also included for extracellular metabolite analysis. b–e Intensities of selected metabolites from late log phase, “In” represents intracellular, “Ex” represents extracellular, and “N.D.” represents not detected. The intracellular intensity signal was collected using the cell pellet from a 1 mL cell culture, and the extracellular intensity signal was collected using 20 μL of the corresponding supernatant. f Structure of XylE (PDB ID: 4GBY)⁴⁸ with residues of ALE-derived mutation locations labeled in green. d-xylose is shown in black ball-and-sticks. g Growth curve of strain LC111 compared to JE3692 on M9 medium supplemented with 30 mM xylose. Source data are provided as a Source Data file.