Table 1 Strains in this study

From: Muconic acid production from glucose and xylose in Pseudomonas putida via evolution and metabolic engineering

Strain

Genotype

References

CJ522

P. putida KT2440 ΔcatRBC::Ptac:catA ΔpcaHG::Ptac:aroY:ecdB:asbF ΔpykA::aroG-D146N:aroY:ecdB:asbF ΔpykF Δppc Δpgi-1 Δpgi-2 Δgcd

3

JE3226

P. putida KT2440 ΔhsdR::Ptac:BxB1int-attB Δgcd ΔampC::PxylE*xylE:xylAB:tktA:talB

35

JE3692

JE3226 ∆gcd::araE-araCDABE

35

QP328

CJ522 ΔhexR ΔampC::PxylE*xylE:Ptac:xylAB:talB:tktApgi-1::pgi-1 PP_1736-1737(intergenic)::Plac:ubiC-C22

This study

QP478

QP328 xylE-A62V, A455V PPP_2569 G→A duplication of PP_5050–PP_5242

This study

LC041

JE3226 ∆pgi-1

This study

LC061

QP478 restoration of G→A at PPP_2569

This study

LC078

QP478 restoration of xylE-A455V

This study

LC093

QP478 restoration of xylE-A62V

This study

LC091

QP328 xylE-A62V, A455V

This study

LC092

QP328 PPP_2569 G→A

This study

LC100

LC091 PPP_2569 G→A

This study

LC111

JE3692 xylE-A62V, A455V

This study

LC147

LC100 ΔpykF::Plac:gpmI

This study

LC150

LC100 ΔpykF::Ptac:maeB

This study

LC151

LC100 ΔpykF::Ptac:rpiA

This study

LC168

LC100 ΔpykF::Ptac:aroK:aroB

This study

LC171

QP478 ΔPP_5050–PP_5242

This study

LC173

QP478 ΔPP_5084–PP_5242

This study

LC199

LC100 ΔpykF::Ptac:aroK

This study

LC224

LC100 ΔpykF::Ptac:aroB

This study

LC345

JE3226 ∆pgi-2

This study

LC347

LC041 ∆pgi-2

This study

LC349

QP328 ΔpykF::Ptac:aroB

This study

  1. *Represents mutation in promoter of xylE35.