Fig. 5: Novel 3’ processing programs in the intestinal stem cell (ISC) lineage.
From: Diverse cell-specific patterns of alternative polyadenylation in Drosophila
a Schematic for differentiation of self-renewing ISCs into enterocytes or enteroendocrine cells. b UMAP of the ISC lineage from intestinal FCA data. c Example of a gene (SCCRO) that undergoes 3’ UTR shortening as ISCs differentiate. d Example of a gene (CG31995) that undergoes distal-to-proximal ALE switching as ISCs differentiate. Note in both examples (c, d), neurons do not dominantly express the isoforms found in ISCs, suggesting this reflects a distinct mechanism to adjust 3’ isoform choice in ISCs. e Global tandem 3’ UTR lengthening in ISCs (n = 73). f Global distal ALE isoform usage in ISCs (n = 8). The boxes are shown on the left side of the panels represent the interquartile ranges, the center lines represent medians, and the whiskers denote the ranges of minima and maxima. *P < 0.05, **P < 0.01, ***P < 0.001 compared with ISC (Wilcoxon test) (g, h) Overlap analysis amongst neurons, spermatocytes and ISCs confirms that the cell-specific programs of tandem APA (g) and ALE-APA (h) are substantially distinct across these settings.
